[Package Specification] Plastic bottle, 500g/bottle

[Performance] White crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3±1 (5% aqueous solution, g/ml,), solubility in water ≥60% (g/ml, at 25℃). Stable at room temperature. The main content of tri-potassium EDTA ≥99.0%. See the product test report for other indicators.

[Application] This product is specially used for in vitro anticoagulation of blood, and is an anticoagulant additive for clinical blood test. It is used for blood sample pretreatment (anticoagulation treatment) in the process of clinical blood collection and test.

[Scope of application] EDTA salt can protect the cellular components of the blood, does not affect the white blood cell count and size, has the least effect on the red blood cell morphology, and can inhibit the aggregation of platelets, suitable for general hematological tests. Except for the isolation and examination of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase and leucine amino peptidase and PCR test. 

The international committee for standardization of hematology (icsh) recommends the preferred anticoagulant for blood counts and classification of tripotassium EDTA. Compared with dipotassium EDTA, it has higher solubility and faster anticoagulation rate.   

[Method of use] the product is dissolved with deionized water (distilled water), prepared into a solution with a certain concentration and content, and then added into the blood collection tube.  

I. Solution preparation method and procedure:

1. Preparation: electronic balance (Accurate to1/10,000), volumetric bottle (100ml, 200ml, 500ml, 1000ml, as required), beaker, deionized water (distilled water), glass rod, washing bottle, etc.

2. Weighing: accurate weighing of certain pre-determined tripotassium EDTA powder by electronic balance, accurate to 1%;  

3. Dissolution: This product is dissolved with distilled water and deionized water. Pour the tripotassium EDTA powder into the volumetric bottle or beaker with part of distilled water (deionized water) before stirring. Carefully pour the powder attached to the mouth and wall of the volumetric bottle or beaker into the bottle or cup with the washing bottle. Especially pay attention to the volumetric bottle and beaker to the cleaning of the volumetric flask and beaker without impurities, oil stains and oxides. Please refer to the relevant contents in the operator's Manual for the cleaning of volumetric flask and beaker. 

When dissolving with beaker, pay special attention to the control of water consumption in the process of dissolving, and reserve enough volume for EDTA tripotassium on the later cleaning glass rod, beaker and other tools, otherwise it will cause over capacity and reduce tripotassium concentration.

4. Constant volume: After all tripotassium EDTA is dissolved, the eye and the scale line of the volumetric flask shall be kept horizontal. Use the washing bottle to slowly add deionized water along the wall of the volumetric flask mouth, and wait until the concave liquid level bottom and the scale line are on the same line.

5. Sufficient mixing: Seal the opening of the volumetric flask with the volumetric cork, hold the upper part of the volumetric flask with one hand and press the cork with fingers, hold the bottom of the volumetric flask with another hand, and mix it upside down carefully for 5-8 times.

6. Inspection and confirmation: Carefully check the key factors such as the weight and volume of the tripotassium EDTA, recalculate the tripotassium EDTA content, and confirm by the supervisor.